Effects of Microbial Fertilizer on Soil Fertility and Alfalfa Rhizosphere Microbiota in Alpine Grassland

Chemical fertilizers are gradually being replaced with new biological fertilizers, which can improve the soil and microorganisms. In this experiment, leguminous forage (Medicago sativa cv. Beilin 201) was used as research object. By measuring alfalfa root systems properties using high-throughput sequencing technology, we investigated effect of (rhizobial) fertilizer at different concentrations on fertility rhizosphere microbiota in alpine grasslands. The results demonstrated that treatment biofertilizer significantly reduced total nitrogen (TN) organic carbon (TOC) content soils, increased densities, number nodules alfalfa. There were differences response microorganisms to biofertilizer, led pronounced changes microbial community structure. abundance beneficial bacteria such Rhizobium, Arthrobacter, Pseudomonas increased. Pearson correlation analysis showed moisture conductivity positively correlated observed richness (p < 0.05). Meanwhile, Actinobacteria a positive nitrate, TOC, TN 0.01). These indicated biofertilizers enhanced altered grassland.